Academic Research Journal of Biotechnology
 

 Academic Research Journal of Biotechnology. ISSN: 2384-616X  Vol. 6(1), pp. 1-7, October, 2018.

DOI: 10.14662/ARJB2018.038

 ©2018 Academic Research Journals

Full Length Research

Evaluation of beetles Scarabaeus Sacer Derived-Chitosan, Anti-Cancer and Anti-bacterial Potentials: In Vitro Study

Amr Abdelkader A.^1*, Mohamed Maged^1**, Aly Fahmy Mohamed^2***

^1,2Faculty of Biotechnology, MSA university

³Head of International Center of Advanced Researches (ICTAR-Egypt) Cairo, Egypt.

Amr.abdelkader@msa.edu.eg * mmglal@msa.eun.eg ** Fahmy.aly@gmail.com  /ictaregysearch@gmail.com ***

Accepted 26 September 2018

Lung and colorectal cancer represent a major health problem all over the world, in addition, bacterial infections impose a serious medical and health concern. Thus, in this study, we evaluate the cytotoxic effect, anti-cancer and anti-bacterial properties of beetles derived chitosan. The study conducted using lung (A549) and colorectal (HCT-116) cancer cell lines to identify the anti-cancer effect and antimicrobial activity against gram-positive bacteria (Streptococcus pyogenes, streptococcus Aureas) and gram-negative bacteria (Salmonella typhimurium). Cytotoxicity was evaluated by describing and measuring recoding morphological changes. The viability and related IC50 were cell type and concentration dependent. Also, related cell apoptosis was monitored using PI stain where early and late apoptosis of treated A549 cells was significantly elevated than in case of HCT-116 cell line (P<0.05).  In the meantime, the necrosis % of treated cells didn't perform any changes between the two cell lines but significantly elevated than that of cell control (P<0.05). Apoptotic profile was examined and showed up regulation of both pro-apoptotic genes (P53 and Bax) accompanied with down regulation of BCL-2 in both cell lines. Also, gram positive bacteria showed a remarkable enhancement by beetles' chitosan unlike gram negative bacteria that showed a lower respond to chitosan anti-microbial property.

Keywords: Beetles' chitosan, toxicity, cell cycle, apoptosis, anti-cancer, anti-bacterial.

Cite This Article As: Amr, A.A., Mohamed, M., Aly, F.M (2018). Evaluation of beetles Scarabaeus sacer derived-chitosan, Anti-cancer and Anti-bacterial potentials: In Vitro Study. Acad. Res. J. Biotech. 6(1): 1-7