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Academic Research Journal of
Agricultural Science and Research |
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Academic Research Journal of Agricultural Science and Research Vol. 3(6), pp. 111-120. June, 2015. ISSN: 2360-7874 DOI: 10.14662/ARJASR2015.018
Full Length Research Genetic and Biochemical Basis of Growth Variation in Bred Clarias gariepinus (Burchell 1822)
*1Oyebola O.O.; Kajopaye A.K. 2 and Awodiran M.O. ³
1Department of Aquaculture and Fisheries Management, University of Ibadan, Ibadan, Nigeria 2Department of Animal Production, Olabisi Onabanjo University, Ago-Iwoye, Nigeria 3Department of Zoology, Obafemi Awolowo University, Ile-Ife, Nigeria *Correspondence email: olusegun.oyebola@yahoo.com
Accepted 24 April 2015
Challenges of
size variants (shooters and runts) in bred Clarias gariepinus
could be solved if relevant information on the basis of the
heterogeneity is known. Genetic and biochemical basis of growth
variation in C. gariepinus was investigated in this study. Tissue
samples of four fastest growers- S (4.16±2.40g)) and least four growers-
R (0.59±0.83g) in a 6weeks old population of bred Clarias gariepinus
were subjected to Sodium Dodecyl Sulphate Polyacrylamide Gel
Electrophoresis (SDS-PAGE). Bands were analyzed for differentiating
locus for S and R. These loci were compared with kDa ladder to determine
molecular weight and biochemical implications. Variants’ genotypes were
analyzed for phylo-genetic relationship using cluster analysis. At <14.7
and >150kDa loci, S and R were differentiated. These loci correspond
with α-amylase inhibitor and polymorphic Prostatic Growth Factor-1
(PGF-1) locus respectively. At 0.75 coefficient of similarity, genotypes
grouped to two major clusters. All R-group members and one of S-group
members were on a cluster while all other S-group members formed another
cluster. In the studied C. gariepinus population, growth
heterogeneity has genetic and biochemical basis. Fast growers were more
genetically versatile. Obtained knowledge is important in discrimination
and utilization of fast growers, Marker Assisted Selection (MAS) and
breeding programmes planning. How to cite this article: Oyebola OO, Kajopaye AK, Awodiran MO (2015). Genetic and Biochemical Basis of Growth Variation in Bred Clarias gariepinus (Burchell 1822) . Acad. Res. J. Agri. Sci. Res. 3(6):111-120.
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